| 발표일자: |
2013년 4월 20일(토) ~ 21일(일) |
| 발표시간: |
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| 발표번호: |
P(e-poster)-079 |
| 발표장소: |
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| Pharmacokinetics and safety of simvastatin using intravitreal delivery routes in mice |
| 안과학교실, 경상대학교 의학전문대학원(1), Baylor College of Medicine(2) |
| 정인영(1), Dennis Y. Tse(2), 박종문(1), Samuel M. Wu(2) |
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목적 : The objective of this study is to determine retinal toxicity and pharmacokinetic profile after intravitreal injection of Simvastatin in mice. Simvastatin is a potent statins that has been shown to protect retinal ganglion cells (RGCs) from degenerative insults and some promising effects in several ocular disease models such as diabetes and PVR.
방법 : Simvastatin (1ul of 2mM) was injected into the vitreous of the right eye of the mice, and the vehicle solution was injected into the left eye as a control. Simultaneous bilateral dark-adapted electroretinography (ERG) was performed 1, 3 and 7 days following the injection. High-performance liquid chromatography (HPLC) of the isolated mouse retina was used to determine drug concentrations at 1,3, 6, 12, 24, 48 hours after injection. Retinal histologic examination was done by electron microscope.
결과 : The amplitude and kinetics of the ERG a- and b-wave exhibited no statistically significant reduction in the all eyes stuied compared to the control eyes. There was exponential decay with a half-life of 2.4 hours after injection with 2 mM simvastatin. Histologic studies revealed normal retinal morphology and structures in all eyes.
결론 : Intravitreal injection of 2mM Simvastatin is safe in the mice, as the retinas show no sign of dysfunction days after injection. The half-life of Simvastatin was short in the retina, and modification of the delivery method would be required to extend the action duration for experimental and clinical applications.
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