| 대한안과학회 제109회 학술대회 발표 연제 초록 |
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| Elevated intraocular pressure causes inner retinal dysfunction prior to cell loss in a mouse model of experimental glaucoma | |||||||||
| 안과학교실, 경상대학교 의학전문대학원(1), Baylor College of Medicine(2) | |||||||||
| 정인영(1,2), Benjamin J. Frankfort(2), Samuel M. Wu(2) | |||||||||
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목적 : To assess the relationship among intraocular pressure (IOP), histology and retinal function changes in a mouse model of induced, chronic, mild ocular hypertension. 방법 : IOP was elevated experimentally via anterior chamber injection of polystyrene beads and measured twice weekly with a rebound tonometer. Histology was assessed with a combination of neurobiotin (NB) retrograde labeling of retinal ganglion cells (RGCs) and TOPRO3 dye. Retinal function was assessed with serial dark-adapted electroretinograms (ERGs) optimized for detection of the a-wave, b-wave, and positive and negative scotopic threshold responses (pSTR, nSTR). Comparisons between bead-injected and saline-injected (control) eyes were conducted. 결과 : Elevated IOP results in a mild, progressive reduction of RGCs at 6 and 12 weeks after bead-injection, but the magnitude of RGC loss is not correlated with cumulative IOP exposure. RGC light sensitivity, as measured by the pSTR amplitude, decreases shortly after IOP elevation, prior to histologic loss of RGCs, and is associated with cumulative IOP exposure. Diffuse changes in retinal sensitivity including an increase in b-wave amplitude occur in parallel and are detected shortly after IOP elevation. 결론 : Polystyrene bead injection results in a mild, chronic elevation of IOP that recapitulates several critical aspects of human glaucoma and results in early changes in retinal electrical function that precede inner retinal cell loss. These changes in retinal function appear to occur in a manner which is related to cumulative IOP exposure. It is possible that glaucoma associated with elevated IOP involves the early disruption of a complex combination of retinal synapses. |
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