| 발표일자: |
2012년 11월 02일 (금) |
| 발표시간: |
15:27~15:34 |
| 발표번호: |
녹F-17 |
| 발표장소: |
A방 |
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| 시신경절단모델에서 Resveratrol 의 신경보호효과 |
| 서울대학교 의과대학 안과학교실, 보라매병원, 서울대학교병원, 분당서울대학교병원 |
| 김석환, 박주현, 김유정, 정진욱, 김태우, 박기호, 김동명 |
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목적 : This study aimed to investigate the neuroprotective effect of resveratrol in an optic nerve transection (ONT) model and to identify the neuroprotective mechanism of resveratrol in retinal ganglion cells (RGC).
방법 : Optic nerve transection and retrograde labeling were performed in Sprague-Dawley rats. Various concentrations of resveratrol (1, 3, 10, 30, and 100 ng/ul) were injected intravitreally immediately after ONT. The number of labeled RGCs was determined at 1 and 2 weeks after ONT. The effect of resveratrol and sirtinol co-injection was investigated. RGC-5 cells were cultured and treated with staurosporine to induce differentiation. MTT assay was performed to evaluate the effect of resveratrol on RGC-5 cell survival under serum-free conditions.
결과 : A dose–response relationship was observed between resveratrol and RGC survival. A single intravitreal injection of resveratrol was neuroprotective in RGCs at 1 week after ONT (p < 0.01). Repeated intravitreal injection of resveratrol showed a neuroprotective effect at 2 weeks after ONT (p < 0.01). However, co-injection of resveratrol and sirtinol diminished the neuroprotective effect of resveratrol (p < 0.05). The neuroprotective effect of resveratrol was observed in RGC-5 cells under serum-free conditions, and sirtinol diminished this neuroprotective effect.
결론 : Resveratrol exerts its neuroprotective effect on RGCs via activation of the SIRT1 pathway in an ONT model. This finding demonstrates the therapeutic potential of resveratrol in treating optic nerve diseases.
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